Bacterial flora associated with the giant freshwater prawn Macrobrachium rosenbergii, in the hatchery system

The objective of this study was to understand the microbial flora associated with the hatchery system of giant fresh water prawn, Macrobrachium rosenbergii during an entire rearing cycle. Bacteriological and physico-chemical analysis was done for different samples of water, larvae, and Artemia. The total bacterial load in well water, seawater and inlet water varied from 10¹ to 10⁵ cfu ml^− 1 with higher counts seen in larval rearing tank (LRT) water. The Vibrio count ranged between 10¹ to 10³ cfu ml^− 1. Larval samples harboured a bacterial load of 10⁶ to 10⁷ cfu/10 larvae. The bacterial load in Artemia hatching water ranged from 4.90 × 10⁴ to 5.63 × 10⁶ cfu ml^− 1 while Artemia had a load ranging from 1.08 × 10⁷ to 2.09 × 10⁹ cfu g^− 1. Vibrio count in the LRT water ranged from 10¹-10³ cfu ml^− 1 while the count in larvae ranged from 10² to 10⁴ cfu/10 larvae. The bacterial genera were predominantly Gram-negative and comprised of Aeromonas spp., Pseudomonas spp., Vibrio spp. and Bacillus spp. and non-spore formers (NSF) were the dominant Gram-positive bacteria. This study documents the bacterial flora associated with Macrobrachium hatchery system during a regular normal run. Knowledge of the qualitative and quantitative aspects of bacterial flora in the hatchery would help to understand disturbances, if any, brought about during disease outbreaks.     

全人工饲料培育施氏鲟苗种试验

为提高施氏鲟的开食率和苗种成活率,在流水条件下,选取刚孵化出膜、体质健壮的施氏鲟仔鱼1000尾,投喂人工配合饲料进行强化开口试验,观察其摄食习性、生长与形态发育变化。结果表明:在16～25℃水温条件下,经100d饲养,平均全长达28.3cm,平均体重达69.7g,并获得了的86.7%开口成活率和73.4%的最终成活率。     

人工饲养对黄喉拟水龟繁殖力的影响

在自然温度，人工环境及工人饲养条件下，黄喉拟水龟可以自然产卵，产卵高峰期在5－6月份，产卵时间有逐年集中并前移的趋势，饲养2年半中，产卵次数，产卵量及受精率逐年提高，产卵量增长了134％，受精率提高了7．8％，产卵次数增加到99％，产卵高峰期，受粗率,高峰期与日粮需求高峰期时间一致，环境的适应及饲料的质和量是决定繁殖力高低的主要因子。     

Effects of temperature and salinity on the reproductive success of Arctic charr, Salvelinus alpinus (L.): egg composition, milt characteristics and fry survival

The effects of temperature and salinity on the reproductive success of Arctic charr, Salvelinus alpinus (L.), were examined by holding broodstock under the following conditions from mid‐May until the end of September: fresh water at ambient temperature (NFW; 8–16 °C); salt water (25–30‰) at ambient temperature (NSW; 4–10 °C); fresh water cooled to saltwater temperature (CFW; 4–10 °C); or salt water heated to freshwater temperature (HSW; 8–16 °C). The relative fecundity of females was similar among groups (P > 0.05; 2685 ± 706 eggs), but females reared in NSW produced significantly larger eggs than those raised in NFW. The highest spermatozoa concentrations were found in milt from males reared in SW and the highest milt glucose concentration was from males kept under coldwater conditions (CFW, NSW). Eggs from NSW and HSW females contained more proteins than eggs produced by NFW females. Eggs from NSW females also contained 40% more lipids than was observed in the other groups, and total energy content was 27% higher in eggs from NSW females than in eggs from NFW females. When FW was cooled (CFW), females produced eggs with protein contents similar to those in NSW, but the lipid contents remained 30% lower. Finally, the best survival at the eyed stage and at hatch was observed in families produced by NSW broodstock. Intermediate values were observed in families from NFW or CFW while the highest mortality occurred in families from the HSW group. All these results suggest that, under the experimental conditions used in the present study, coastal seawater conditions offered the most favourable summer rearing conditions with respect to the reproductive success of Arctic charr.     

紫红笛鲷人工繁殖及育苗技术研究报告

利用海水土池或网箱养殖三龄以上的紫红笛鲷成鱼,于催产前三个月进行强化培育。在水温达到 20.0℃以上的春、夏季,用绒毛膜促性腺激素(HCG)和释放激素(LRH-A_(2-3))进行催产,混合或单一使用,剂量视亲鱼性腺成熟度而定。受精卵在盐度27.9-33.5,水温26.5-30.5℃,pH8.2的水环境中经15-17h孵化。开口仔鱼在土池水温27.2-35.5℃.盐度:23.5-35.6.pH8.0-9.5的条件下,经28-30d培育.鱼体全长为2.5-3.2cm,仔鱼变态完全,成为幼鱼。     

美洲鳗鲡苗种的培育技术

总结了自1996年以来美洲鳗鲡(Auguilla restrata)苗种的培育。从鳗苗的选购、放苗前的准备工作、鳗苗人池放养、饲养管理、疾病防治和配合饲料转换等6个方面探讨美洲鳗鲡苗种培育技术。     

九孔鲍北方青苗及生长的探讨

探讨了九孔鲍(Haliotisdiversicolorsuertexta)在北方自然繁殖季节育苗的条件、发育时间和生长速度。2004年8月1日,用紫外线照射海水诱导产卵和人工授精的九孔鲍受精卵,经过60天培育,获6～12mm的幼鲍50万只。     

Estimation of flow in a rearing tank of marine fish larvae by simplified numerical computation—a case of two-dimensional flow

Marine fish larvae are fragile against physical stress. However, few studies have been conducted to evaluate the flow field in a rearing tank, which is assumed to provide a high degree of physical stress to marine fish larvae. The flow field in a rearing tank (volume of 1 m³) is generated by aerators, which are commonly used to provide oxygen.

This paper is a report on the estimation of stationary flow in the rearing tank of marine fish larvae. The larvae are seven band grouper larvae of Epinephelus septemfasciatus, which have a very low survival rate immediately after the hatching of eggs. The experiments of rearing of seven band grouper larvae were carried out using rearing tanks with four aeration rates (1000, 200, 50 ml/min, and no aeration). The effects of aeration on the survival and floating death of seven band grouper larvae were examined. The experiments confirmed that the mass mortality of seven band grouper larvae depends on the flow rate in the rearing tank. Aeration at 200 ml/min resulted in the highest survival and growth rates of grouper larvae.

Larvae-rearing experiments provided evidence that the flow rates of the rearing tanks are very important design aspects of rearing tanks. The estimation of flow in a rearing tank for an aerating rate of 200 ml/min was carried out by numerical calculation. The computation was simplified by a two-dimensional flow based on experimental results. The calculated flow in the rearing tank was compared with the experimental one. The calculation of the stationary flow in the rearing tank showed good qualitative and quantitative agreement with the experimental results. The numerical estimation of the flow in a rearing tank of marine fish larvae was confirmed to be effective and satisfactory for the design of a tank that would provide optimum performance.     

Nutritional evaluation of decapsulated cysts of fairy shrimp (Streptocephalus dichotomus) for ornamental fish larval rearing

The suitability of decapsulated cysts of the fairy shrimp, Streptocephalus dichotomus as a sole diet was evaluated for the ornamental angelfish Pterophylum scalare larvae. Brine shrimp, Artemia sp., and microworms, Panagrellus redivivus, were used as comparison foods. The results indicate an appreciable weight gain of 15.22±1.69 mg and a growth (length) of 0.876±0.03 cm in experimental fish fed decapsulated fairy shrimp cysts compared with the growth and weight of fish fed other foods. The influence of the experimental diets was further reflected in the composition of fatty acid and amino‐acid profiles of the experimental fish. Angelfish larvae readily consumed the decapsulated cysts and utilized them efficiently during the early days of exogenous feeding.     

Models for comparing average first-year growth in length of freshwater fish

Abstract  Logistic curves of the average growth in length were elaborated for young-of-the-year perch, Perca fluviatilis L., zander. Stizostedion lucioperca (L.), ruffe, Gymnocephalus cernua (L.), bream, Abramis brama (L.), and roach, Rutilus rutilus (L.), using data from 62 localities. Mean relative deviation from this average growth (MRDAG) is proposed as a simple and reliable indicator of growth rate. The MRDAG for perch, zander, bream and roach showed a significant negative linear relationship with the geographical latitude.